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Protocols examining GH deficiency models, age-related GH decline, and hypothalamic-pituitary axis integrity use both GHRH and ghrelin receptor agonism together to characterize the full secretory capacity of the pituitary

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Thermal shift assay CBS constructs were diluted in thermal shift buffer (25 mM HEPES, pH 7.5, 200 mM NaCl, 2.0 mM TCEP) to 0.3 mg/ml with SYPRO-Orange (Invitrogen) diluted 1000X in a total volume of 20 l