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2.5 Affinity isolation of HPX binding proteins We used well-controlled affinity chromatography to isolate putative hemopexin receptors (HPXRs) from whole cell extracts (100,000 x g isolates in HEPES buffered pH 7.4 Triton X-100 0.5%, 150 mM NaCl containing phosphatase and protease inhibitors, Millipore Sigma) of non-hepatic human promyelocytic HL-60, HepG2 cells, and freshly plated primary human hepatocytes

(2025) Lancet, 405: 813

Other signs and symptoms : muscle weakness and fatigue, anxiety, heat intolerance, pretibial myxedema

The oxidation of the fusion-constructs was followed by mixing 2 M reduced protein in syringe 1 with variable concentrations of GSSG in syringe 2

Reduction of selenium- binding protein 1 sensitizes cancer cells to selenite via elevating extracellular glutathione: A novel mechanism of cancer-specific cytotoxicity of selenite