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Characteristics and number of each group in experiments

Updated July 2023

A 0.1 mM stock solution of DCCD was prepared in 100% dimethyl sulfoxide and added to the designated experimental groups at a 1:10 dilution ratio, yielding a final concentration of 10 M DCCD
The reconstitution process (abbreviated) Swab both vial tops with alcohol Draw calculated water volume into syringe Inject water slowly down vial wall (not onto powder) Wait 30 seconds, then swirl gently (no shaking) Inspect for clarity (clear = good, cloudy = problem) Label vial with concentration and date Refrigerate at 2-8 degrees Celsius Use within 28 days Critical reminders Use bacteriostatic water, never sterile water for multi-dose vials
