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Beyond visual checks, the most reliable indicator is efficacy, if appetite suppression diminishes or weight loss stalls unexpectedly mid-protocol, a degraded vial is a possible cause

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Type III collagen is also cleared promptly and substituted with collagen type I fibers starting at the 14th day of treatment (i.e., improved load to failure correlate with the advanced collagen type I distribution) (Figure 10e,f,k,l and Figure 11e,f)