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Subcellular distribution of key metabolites in isolated liver cells from fasted rats

Quantitative real-time PCR was performed using Taqman qRT-PCR probes (Applied Biosystems, Foster City, CA) and normalized to tissue -Actin by the C T method using StepOne Software v2.1 (Applied Biosystems, Foster City, CA)

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Solid-phase extraction-based methods SPE is a preferred method for trapping analytes and separating them from the sample matrix, addressing the disadvantages of LLE such as excessive solvent use and prolonged operation times (Badawy et al., 2022