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In melanoma, LPO products can exert both pro- and anti-tumorigenic effects depending on their concentration, cellular context, and tumor stage [108]

Models utilizing cell cultures and in vitro tests also have their limitations

J Biol Chem 274:1206112066

The expression of target genes was analyzed via quantitative real-time (RT) PCR with a QuantaStudio 5 qPCR machine (Applied Biosystems, Thermo Fisher Scientific)

This, in turn, triggers the formation of a hetero-oligomeric cysteine synthase complex (CSC) with O -acetylserine(thiol)lyase B, a process that activates sulfur assimilation and the accumulation of sulfur-containing metabolites such as GSH (Watanabe et al