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Description
Western blotting Sample preparation Liver tissues were pulverized in liquid nitrogen and 20 mg were weighed and homogenized in ice cold 1X RIPA buffer (50 mM Tris, 150 mM sodium chloride (NaCl), 1% NP40, 0.5% sodium deoxycholate and 0.1% SD, pH 8) using a glass Dounce homogenizer
This happens as they are overworked, and as you age
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Muller FL, Lustgarten MS, Jang Y, Richardson A, Van Remmen H
