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Linker-ligated DNA for each sample was then purified (QiaQuick PCR purification, Qiagen) and amplified for 28 cycles with primers that contained unique 6 nt barcodes, 05 nt of phasing, and Illumina P5/i5 or P7/i7 sequences (Supplementary Table 3)
Association of spermidine plasma levels with brain aging in a population-based study
10.1016/j.biopsych.2015.05.017 Biol
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Cells were grown at 37 C, 8% CO 2 , for 2 days and collected by centrifugation at 5350 g for 10 min
