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Analysis of gene ontology terms using DAVID software revealed a strong enrichment of genes involved in glucose metabolism (glycolysis), angiogenesis, and cell proliferation among the upregulated genes in NSCs from Gpr124 KO cortices, while importantly genes functioning in neurogenesis were most enriched among the downregulated genes (Fig 3B and C)

The current standard operating procedure by the European Center for the Validation of Alternative Methods (ECVAM) recommends the use of MTT reduction as parameter to make prediction on the skin irritancy of substances using EpiSkin TM test method, while IL-1 endpoint is regarded as a useful adjunct to MTT assay 48

For 100 mg vials, 3 mL bacteriostatic water yields 33.3 mg/mL, with 1 mg doses at 3 units (0.03 mL) and 2 mg doses at 6 units (0.06 mL)

Conclusion: Our study suggests that recanalization of conservatively treated patients with sCeAD-related occlusion does not put individuals at risk of ischemia, clinical worsening or poor functional outcome
