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Inactivation of the detoxifying enzyme glutathione -S- transferase has been observed in cells exposed in vitro to busulfan and, because of the inactivation of the enzyme, the level of free electrophilic species in the tissues of the exposed organisms can increase to levels that irreversibly damage the cell, which is deranged to apoptotic or necrotic death

Using the ergodic assumption and equal S phases, tricycle analysis indicated a decrease in both G1 and G2M in holoclones (SCs) (Fig

PMID: 24753527

Certain side effects have been reported in clinical trials, including gastrointestinal discomfort and allergic reactions