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Gently roll the vial between your palms for several minutes until the lyophilized pellet is fully dissolved

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BMC Gastroenterol 11(1):138 Bank S, Skytt Andersen P, Burisch J, Pedersen N, Roug S, Galsgaard J, Ydegaard Turino S, Broder Brodersen J, Rashid S, Kaiser Rasmussen B, Avlund S, Bastholm Olesen T, Jrgen Hoffmann H, Kragh Thomsen M, stergaard Thomsen V, Frydenberg M, Andersen Nex B, Sode J, Vogel U, Andersen V (2014) Polymorphisms in the Inflammatory Pathway Genes TLR2, TLR4, TLR9, LY96, NFKBIA, NFKB1, TNFA, TNFRSF1A, IL6R, IL10, IL23R, PTPN22, and PPARG are associated with susceptibility of inflammatory bowel disease in a Danish Cohort

Instead, slowly and gently depress the plunger, letting the water run down the side of the glass to pool at the bottom