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These findings collectively suggest that an optimal range of maternal one-carbon nutrients is important, where both deficiency (for vitamin D clearly, and B 12 possibly) and extreme elevations (for B 12 /folate) may be detrimental

Human embryonic kidney (HEK)-293T cells were plated at 2535% confluence in Dulbeccos Modified Eagle Medium (DMEM) (Gibco) supplemented with 10% fetal bovine serum (FBS) (Sigma), 200 mM L-glutamine (Lonza), and 10,000 U/ml penicillin/streptomycin (P/S) (1:1, Lonza)

[DOI] [PubMed] [Google Scholar] 300.Kanai Y

Despite several advances in the understanding of lncRNA function, the function of the vast majority of lncRNAs are still unknown

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