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Visceral afferents respond to various mechanical and chemical stimuli [82], including changes in microbiome diversity [83], neuropeptides and hormones secreted by enteroendocrine cells (EECs) [84], as well as sensitization to inflammatory mediators
Doses were determined by ad hoc laboratory analyses for the most frequently consumed additive-food pairs and doses retrieved from other sources such as the European Food Safety Authority after an official request for public access to the document
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The higher dose level additionally produced symptoms of intoxication
