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proposed that cuproptosis is induced by proteotoxic stress caused by an excess of intracellular copper ions

[DOI] [PubMed] [Google Scholar] [5].Hoffmann M, Kleine-Weber H, Schroeder S, Krger N, Herrler T, Erichsen S, et al

The separation was performed using a gradient elution with water mixed with acetonitrile

Nat Rev Mol Cell Biol 8(2):101 Haeggstrom JZ, Funk CD (2011) Lipoxygenase and leukotriene pathways: biochemistry, biology, and roles in disease

Then, the resulting MDA-thiobarbiturate was measured by the absorbance intensity at 540 nm using a UV-Visible spectrophotometer, which compared to the standard curve prepared by tetramethoxypropane