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Samples were filtered (0.45 m) to remove residual cellular debris and loaded onto a HisTrap Column (GE Healthcare) at 4 C

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Both pulsatile and continuous administration of moderate amounts of GH to healthy human volunteers lead to a dose-dependent stimulation of lipolysis as revealed by increased circulating FFA levels, glycerol, and increased lipid oxidation rates

Adherent cells were scrapped, then centrifuged at 4C and 13000 g for 15 min, and the resulting supernatants processed using established protocols.[19] Intracellular levels of 1-MNA and as well as the IS were determined from LC/MS/MS peak areas