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However, it remains possible that subjects could have had a tendency to deliver lower doses when reaching the end of the study

doi: 10.1038/ejhg.2009.187 367 UlrichG.SalvadA.BoersemaP.CalT.FoglieniC.SolaM.et al

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The solvent was added to both the sample and standard solutions, and the mixtures were centrifuged at 13,680 g at 4 C for 10 minutes to obtain the supernatant for subsequent LC-MS/MS analysis

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