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This leads to a number of secondary motor and non-motor symptoms 9,11 such as neuropsychiatric disorders (anxiety and depression), autonomic dysfunction (constipation), sleep abnormalities (insomnia), olfaction and visual disorders, and cognitive decline including dementia 12,13

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APOE and neuroenergetics: an emerging paradigm in Alzheimers disease

The supernatant was dried and added to 5% trichloroacetic acid (TCA) containing 2% 2-mercaptoethanol to reduce the oxidized form of glutathione, and the mixture was used as the deprotenized plasma fraction