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The term porphyria is of Greek origin and meaning purplish pigment, relating to the color of porphyrins

For smFRET binding experiments, single-cysteine substitutions in LpoB (pSI204, Ec LpoB S211C ) and PBP1b (pSI233, Ec PBP1b C794A ) were designed, based on the PBP1b-LpoB AlphaFold prediction, such that the distance between fluorophores would be 30 or less in the complex

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