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Western blot analysis of C-CASP3 protein was consistent with the above results, i.e., the expression level of C-CASP3 was significantly reduced in the peroxynitrite + ebselen group, while it was significantly increased in the peroxynitrite + gpx1 / group compared with the peroxynitrite-only group (Figure 4C)

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8.3 Biomedical investigations and their interpretation 8.3.1 Biochemical analysis 8.3.1.1 Blood, plasma or serum - Sodium, potassium, calcium - Alanine aminotransferase, aspartate aminotransferase - gamma-Glutamyltransferrase, alkaline phosphatase, bilirubin (total and direct), creatine kinase - Creatinine (urea), protein - Glucose, lactate - Optional: -hydroxybutyrate - Dedicated analysis: pyridoxine 8.3.1.2 Urine Qualitative testing for glucose, ketone bodies, sediment, haeme proteins 8.3.1.3 Other fluids No dedicated test 8.3.2 Arterial blood gas analyses pH, pCO 2 , pO 2 , actual HCO 3 - concentration, base excess, O 2 -saturation 8.3.3 Haematological Analyses Count of red and white blood cells, platelets Haemoglobin, haematocrit Differential blood picture Prothrombin time 8.3.4 Interpretation of biomedical investigations In severe cases of INH poisoning metabolic acidosis occurs