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However, it has limitations, including sensitivity to factors like pH and temperature, which can influence the reaction time (Shahidi and Zhong 2015)

Gradient TGX gels (Bio-Rad) with 420% polyacrylamide were used to separate proteins within samples by electrophoresis at 200 V for 30 min

J Radioanal Nucl Chem 327 , 673689 (2021)

In addition, daratumumab has been conjugated to DOTA for radiolabeling with the alpha-emitter actinium-225 ( 225 Ac), showing potent CD38-positive tumor cell-killing in vitro and enhanced anti-tumor activity (~ 30-fold) in vivo without significant side effects [131]

According to the exploration results of the T1D patients or models, the following bacteria were mainly increased in abundance: Bifidobacterium longum (166, 167), Eubacterium rectale (168), Bacteroides uniformis (169), Lachnospira spp., Intestinimonas spp., Micrococcales spp (170)., Actinomyces spp (171)., Bacteroides spp (172)., Prevotella spp (168)., Ruminococcus spp (173)., and Veillonella spp (174)., whereas the bacterium with decreased abundance mainly includes butyrate-producing bacterium, lactic acid-producing bacterium, and mucin-degrading bacterium, such as Lactococcus , Lactobacillus ( 173), Streptococcus (174), Bifidobacterium , Proteobacteria (172), Subdoligranulum , Roseburia , Eubacterium , Anaerostipes (175), FP (176, 177), Clostridium clusters IV (178, 179), and XIVa (180, 181)