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Collapse assay Cos7 cells were plated (1 10 5 cells/ml) onto glass coverslips, and 24 h later, cells were transiently transfected with plasmids encoding for empty vector (pmCherry) or co-transfected with myc-PlexinB1 together with pmCherry-RanWT, pmCherry-RanQ69L, pmCherry-RanT24N or pmCherry using Lipofectamine 2000 as per manufacturers instruction

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The codon-optimized Rg GUS, Bifd GUS, Cp GUS and Bf GUS genes that had been individually inserted into plasmid pUC57 were purchased from GenScript

Bai H., Gu H., Zhou W., et al., PDLike Pathogenesis Induced by Intestinal Exposure to Microplastics: An in Vivo Study of Animal Models to a Public Health Survey, Journal of Hazardous Materials 486 (2025): 136974

Superoxide-mediated oxidative stress accelerates skeletal muscle atrophy by synchronous activation of proteolytic systems