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Protein was eluted with 4CV of 60% buffer B and immediately diluted 10-fold with Buffer A prior to concentration and 3 rounds of buffer exchange to remove excess imidazole by centrifugation at 3095xg at 4 C in 100 kDa cutoff concentrators (Amicon)

Diagnostic tools range from clinical examination and nerve conduction studies (NCS) to magnetic resonance imaging

DOI.org (Crossref) , Gastroenterology , vol

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10.1016/j.freeradbiomed.2018.08.014 [DOI] [PMC free article] [PubMed] [Google Scholar] Rusetskaya N