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pK a determination of water-insoluble drugs in organic solvent-water mixtures
Then, 5 L of 250 mM iodoacetamide was added to alkylate the thiol group of the cysteine residues, and the solution was incubated further at room temperature for 15 min
doi: 10.1034/j.1399-3011.2000.00723.x
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However, long-term safety concerns about a potential association with malignancy have led to restrictions on prolonged use in some countries
