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All the NPT simulations (300 K, 1 atm) were carried out in the Desmond program (Schrdinger, LLC) on graphics processing units (GPUs), with a recording interval of 9.6 ps and the van der Waals and short-range electrostatics cut off at 9

The discomfort may spread and affect daily activities

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To mitigate ethanol accumulation, feeding solutions were added at rates matching the glucose consumption rate, effectively maintaining ethanol levels below 15 g/L while supporting both cell growth and glutathione production