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BSA at 5% was used for pre-incubation at 37C for 30 min, and the sides were incubated with the primary antibodies at 4C for 24 h
It is recognised that it takes approximately 5 half-lives for this to occur
We used adult male Wistar rats (270320 g) maintained on a 12 h light/dark cycle at a temperature of 2224C with free access to food and water
Some individuals report noticeable changes after 48 weeks , although results vary depending on diet, exercise, and metabolism
