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Briefly, primary cells were washed with PBS, lysed in 100 l of 0.4 N perchloric acid (PCA) for 30 min at 4 C, and centrifuged, and the supernatants were neutralized with four volumes of 0.1 M NaH 2 PO 4 and 5 mM EDTA, pH 7.5

All markers are interpreted against local reference ranges and the individual patient

TempO-Seq transcriptome analysis HepG2 cells or PHH were seeded in 96-well plates (20,000 cells/well) and exposed to different concentrations of NFT ranging from 5 to 125 M

Lactate is released by astrocytes through MCTs into the extracellular matrix, from which it may be transported into neurons via MCTs present on neurons or passively through gap junctions (Dienel, 2019

6 ng of cDNA was used for each reaction and all qPCR results were carried out in duplicate or triplicate, along with no template controls and no RT controls where appropriate