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Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH-Px, so the portion of GSH reduction by non-enzymatic reaction should be subtracted

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BPC-157 is a synthetic 15-amino-acid pentadecapeptide commonly discussed in preclinical research as a laboratory tool for studying cell migration, endothelial behavior, nitric oxide-related signaling, and angiogenesis-associated readouts.

Simultaneous detection of both the oxidative and reductive species of redox couples, especially the most representative pair glutathione/hydrogen peroxide (GSH/H2O2), is crucial to accurately map the cellular redox status