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Description
The membrane pellet was resuspended in buffer (20 mM Tris pH 8.0, 150 mM NaCl) and homogenized with the help of a Dounce homogenizer, followed by the addition of 30 mM n-dodecyl--D-maltopyranoside (DDM) and 6 mM cholesteryl hemisuccinate (CHS) for solubilization at 4 C
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These effects tend to go away quickly
Transport into the cells is by simple diffusion followed by rephosphorylation
