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First, the pellet was resuspended in AE buffer 50 mM Na-acetate pH 5.3, 10 mM EDTA, and 1% SDS)

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If oral B12 replacement has been trialled in a person with metformin-associated B12 deficiency, I would suggest that a repeat measurement of B12 levels after 812 weeks is a good time to reassess whether absorption is adequate or whether a switch to intramuscular replacement is required

Nat Nanotechnol 11(11):986994 Zhou F, Mei J, Han X, Li H, Yang S, Wang M, Chu L, Qiao H, Tang T (2019) Kinsenoside attenuates osteoarthritis by repolarizing macrophages through inactivating NF-B/MAPK signaling and protecting chondrocytes