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In contrast, depleting GSH reduced the frequency of long actin tails at 48 h as compared to untreated cells (Fig

Although conceptual and functional overlap exists, this distinction is important because the two categories are subject to different regulatory pathways and evidentiary requirements

After harvesting the cells by centrifugation at 4,000 g for 30 min, the cells were resuspended in lysis buffer (50 mM sodium phosphate, pH 8.0, 300 mM NaCl, 10 mM imidazole) and lysed using a sonication for 1 h with a 10-s pulse mode (10 s on and 10 s off) on ice

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Abstract The fungal pathogen Cryptococcus neoformans is well adapted to its host environment