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Extracts of the medium were incubated at 80 C for at least 15 min before being centrifuged at 15,000 g for 20 min

10.3390/biomedicines10123207 [DOI] [PMC free article] [PubMed] [Google Scholar] 50

It is important to clarify that, whereas population-level PRS models require formal effect-size modeling, linkage disequilibrium correction, ancestry calibration, and validation across large cohorts, the score used in this workflow is a functional polygenic score (PRS-F) designed for mechanistic, individual-level interpretation rather than for epidemiological prediction

It has the capacity to be able to pass through all biological membranes due to its small molecular size and high lipophilicity and it is evenly distributed to all biological tissues and fluids by crossing the bloodbrain barrier [3]

For example, mitochondria isolated from the rat whole brain, when exposed to Aos, produce more reactive oxygen species (ROS) [86]