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Since NSP activity is the core pharmacodynamic indicator for evaluating DPP1 inhibition effects, developing simple and accurate NSP activity detection methods is crucial for clinical research and individualized treatment ( 9.2 Future research directions Development and validation of biomarkers constitutes a key element for achieving precision treatment

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E2 appears to exert anorexigenic effects, whereas P4 in the presence of E2 tends to increase appetite and preference for energy-dense foods, an effect further modulated by changes in leptin, ghrelin, peptide YY (PYY), and possibly central insulin signaling [114]