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HepG2 (HB-8065), 3T3-L1 (CL-173), and C2C12 (CRL-1772) cell lines were sourced from American Type Culture Collection (ATCC) and maintained in DMEM with 10% FBS, 100 units/mL penicillin, and 100 g/mL streptomycin

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IGF-LR3 IGF-1 LR3 (Insulin-like Growth Factor-1 Long R3) is a modified form of IGF-1 with a longer half-life and reduced binding to IGF-binding proteins, allowing for increased bioavailability