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Though short term (48 hours) resistance to MTX seemed to be directly related to cellular proliferation, resistance in longer exposure times (96 hours) was associated with glutathione (GSH) levels

After 20 min of reduction 5 L of 0.1 mol/L BCPB in water was added to each sample to carry out the derivatization reaction

Oxidative phosphorylation (OxPhos)

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Frequent coexpression of MRP/GS-X pump and -glutamylcysteine synthetase mRNA in drug-resistant cells, untreated tumor cells and normal mouse tissues
