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In vivo, HPV infection requires initial association of virus with an acellular basement membrane, and it was shown that antibodies to L1, the major capsid protein of HPV, can interfere with infection at two stages

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962 Downregulating TMPRSS6 using small interfering RNA (siRNA) induces hepcidin expression, reducing tissue and serum iron levels in Hbb th3/+ mice

Fluorescence in situ hybridization (FISH) On the first day, brain slices are washed in DEPC-PBS for 5 min, followed by a 5-min wash in DEPC-PTW (0.1% Tween-20 in PBS), and then incubated in 2 SSC + 0.5% Triton -100 for 30 min