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doi: 10.1038/sj.jid.5700327

The cell cultures were maintained in RPMI-1640 media and supplemented with 10% FBS and 1% penicillin/streptomycin at 5% CO2 in an incubator

An important and balanced perspective

The FDAs response timeline after that is longer months, sometimes longer than a year for final rules

A subset of cultured cells expressed GLP- 1 receptor, and mice with a-cell specific GLP- 1 receptor knockout have mild glucose intolerance and higher glucagon secretion in response to glucose challenges than animals of the wild type, which may indicate that GLP- 1 directly affects this cell [69]
