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Before use, the protein was denatured in 6 M urea and supplemented with 2 mM tris(2-carboxyethyl)phosphine (TCEP, Sigma-Aldrich, Solna, Sweden) at pH 7.4 to obtain complete reduction and dissolution of potential aggregates

HIF-1, Hypoxia-inducible factor 1

bone marrow and TRIS-BPC157 (particularly in a daily dose of 10 g/kg b.w

After incubation with appropriate HRP-tagged secondary antibodies and chemiluminescent substrate (Thermo Fisher, USA), the blots were imaged and processed with a ProteinSimple FlourChem E (Bio-Techne, USA)

Effect of a low-fat fish oil diet on proinflammatory eicosanoids and cell-cycle progression score in men undergoing radical prostatectomy