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Cytokine and chemokine assays Protein was extracted using a 2-cm piece of distal colon and homogenized in 300 l of Meso Scale Discovery lysis buffer with protease and phosphatase inhibitors as described in the assay protocol

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1 Aged Muscle Stem Cell Rejuvenation Neelakantan et al., Biochemical Pharmacology (2019) In a follow-up study, the authors tested whether NNMT inhibition could reverse the age-related decline in muscle stem cell (satellite cell) function

Aim to drink at least 1.5 litres of water per day, and even more during hot weather or when exercising