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For invasion assays, after the scratch procedure, cells were overlaid with 50 L of 2 mg/mL collagen type I prepared in HEPES-buffered (25 mM, pH 7.4) DMEM high glucose with 20% FBS
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Although there are many effective ways to treat cardiovascular diseases, it is still one of the leading causes of death worldwide ( The classic product trimethylamine oxide (TMAO) is a phospholipid metabolite related to intestinal microorganisms and is closely related to people's everyday diet ( Hazen et al