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Ha et al., 1999)

After 24 h, cells were lysed and the luciferase activity was assayed in the lysates using Promegas Dual-luciferase assay system according to the manufacturers protocol (Promega, Madrid, Spain)

CFB is also part of the CIBER-Consorcio Centro de Investigacin Biomdica en Red (CB 2021), Instituto de Salud Carlos III, Ministerio de Ciencia e Innovacin, and Unin EuropeaNextGenerationEU

Therefore, the success of cereblon-based PROTACs is due to a set of key factors: Firstly, the most commonly used cereblon ligands are IMiDs, as they exhibit highly specific, strong and biophysically validated E3 ligase binding affinity 43

Hereby, zinc acts in a direct manner by binding reversibly to regulatory sites in signaling proteins, or indirectly by influencing enzymes such as phosphatases which are a component of and regulate signaling pathways [45]