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Plant-mediated VIGS involves using Agrobacterium transformation to infect plants with Tobacco rattle virus (TRV) that carries double-stranded RNAs targeting an aphid gene

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All mice were fed standard rodent chow (PicoLab 5053, LabDiet) and housed in a specific pathogen-free facility at a controlled temperature of 2223 C on a 12-h lightdark cycle

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