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Description
Here, we describe the GST-His method as a new small-scale affinity purification system for recombinant proteins, based on a N-terminal Glutathione Sepharose Tag (GST) 2,3 and a C-terminal 10xHis tag 4 , which are both fused to the protein of interest
Precision begins with preparation
translation to clinical veterinary dosing remains investigational
Further, factors like age and kidney function can affect B12 excretion and may influence the recommended dosing frequency
Fasano A (2012) Leaky gut and autoimmune diseases
