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2.4 Analysis of index measurements 2.4.1 Liver histomorphometric analysis Liver tissues of 0.5 cm3 each were removed from Bournes fixative, dehydrated with different concentrations of alcohol using a biological tissue automatic dehydrator (KD-TS3A), paraffin-embedded tissues, trimmed the solidified wax blocks, and sliced with a tissue slicer (model: RM2235 slicer produced by leica, Graaf, Germany) with a thickness of 6 m

Skin Wheal After satisfactory identification of the proximal sciatic nerve, widely prep the skin with chlorhexidine, allow to completely dry, and place 23 mL lidocaine skin wheal, 23 cm cephalad, or caudad to the transducer