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Benefit #3: Improved Insulin Sensitivity (Strong Evidence) Evidence Quality: Multiple animal studies with human relevance Mechanism: Enhanced glucose metabolism through NAD+ restoration 5-Amino-1MQ significantly improves insulin sensitivity through metabolic optimization

In situ hybridization In situ hybridization (ISH) was performed on fresh frozen coronal brain sections (25 m) according to protocols that are available at Briefly, digoxigenin (DIG)-labeled RNA probes were transcribed in vitro from cloned cDNAs for mouse Pdgfra, Enpp6, Plp, Mbp and Mog as previously described (Huang et al., 2018