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Four h after transfection, the culture was trypsinised and 1.5 10 4 cells in fresh medium were transferred to optical quality 96-well plates (PerkinElmer Cell Carrier Ultra 96-well) and imaged the following day

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The pharmacological actions of Danzhi-Xiaoyao-San on depression involve lysophosphatidic acid and microbiota-gut-brain axis: Novel insights from a systems pharmacology analysis of a double-blind, randomized, placebo-controlled clinical trial

However, an optimal gene copy number should be considered because of metabolic burden by protein overexpression ( sod -lc gene expression were constructed under promoter #14 (Figure 3A), which one to four copy number recombinants named as S-3/pKLH167, S-3/pKLH337, S-3/pKLH341, and S-3/pKLH344