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CD44 expressed by fibroblastic reticular cells in secondary lymphoid organs regulates trafficking of dendritic cells, and thus has an essential role in the priming of T cells and the adaptive immune response.

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To expand on the chemistry of attaching ligands to agarose beads a little, there are usually 3 components to the ligand (Figure 3): the ligand itself that binds to the peptide or protein tag the reactive chemical group that forms a covalent bond with the ligand a spacer that connects the reactive chemical group to the agarose bead Figure 3