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72 FengC.YangM.LanM.LiuC.ZhangY.HuangB.et al (2017a)

Immunoprecipitation of Target Antigen 3.1 Remove the tubes from the magnetic separator and add your sample containing the antigen (Ag) (typically 5-50 g in 400 L binding/wash buffer) and gently pipette to resuspend the Protein A/G Magnetic Beads-Ab complex

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These systems include a) enzymatic defences such as catalase, superoxide dismutase and glutathione that inhibit the formation of toxic OH - by metabolizing superoxide, H 2 O 2 and lipid peroxides, and b) entrapping free radical defences such as histidine-peptides, the iron-binding proteins transferring and ferritin, dihydrolipoic acid, reduced CoQ 10 , melatonin, urate and plasma protein thiols [24, 191-193]