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The pressure of ADT leads to AR-activating alterations in androgen levels, the AR gene, and the elaborate and diverse molecular machinery that AR needs to engage to alter transcription of its target genes

Lipid extraction was performed using a modified Bligh-Dyer method 66 Specifically, each cell pellet was re-suspended in 0.8 mL of 1 PBS (phosphate-buffered saline), and transferred into a 17 mL glass tube with a Teflon-lined cap, which is then followed by the addition of 2 mL of methanol and 1 mL of chloroform to create a single-phase Bligh-Dyer solution (chloroform/methanol/PBS, 1:2:0.8, v/v/v)

doi:10.1007/s13555-014-0046-1

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The importance of an aromatic residue at this position is further underlined by the fact that ~70% of non-olfactory class A GPCRs contain a W 6.48 and ~16% a F 6.48