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Luciferase activity was assessed using the Dual-Luciferase Reporter Assay System (Promega) 72 h post-transfection with CBS-3UTR-WT or CBS-3UTR-Mut
These processes leading to neuronal decline during senescence will also contribute to the pathophysiology of neurodegenerative processes (Wang et al., 2019)
While buffer systems typically may need optimization for individual proteins, we recommend PBS as initial starting buffer for teabag purification for secreted proteins
Hear Res 257 , 815, (2009)
Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature
