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The gradient separation was accomplished at flow rate of 0.35 ml.min 1 and temperature 45C with mobile phase composed of solvent A (0.5 mM ammonium acetate, acetic acid 0.001% v/v) and solvent B (methanol:acetonitrile 75:25 v/v mixture with 0.5 mM ammonium acetate and 0.001% v/v acetic acid)

Thus, cultural practices that improve the competitive ability of the existing turf and/or promote the establishment of a new stand are the best methods of purslane management, prevention, and control

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